Powerful and versatile tool for vibrational spectroscopy and chemically selective imaging:

• Label-free optical imaging
• Minimally invasive technique
• Non-photobleaching signal
• 3D sample imaging capability
• NIR pump and Stokes wavelengths suitable for deep-tissue imaging
• Picosecond pulse duration – good compromise between efficiency and spectral resolution

Coherent anti-Stokes Raman scattering (CARS) spectroscopy primarily was used in chemistry, physics and related fields. It is sensitive to the same vibrational signatures of molecules as seen in Raman spectroscopy, typically the nuclear vibrations of chemical bonds. Unlike Raman spectroscopy, CARS employs multiple photons to address the molecular vibrations, and produces a signal in which the emitted waves are coherent with one another. As a result, CARS is orders of magnitude stronger than spontaneous Raman emission. CARS is a third-order nonlinear optical process involving three laser beams: a pump beam of frequency ω _pump , a Stokes beam of frequency ω _Stokes and a probe beam at frequency ω _probe . These beams interact with the sample and generate a coherent optical signal at the anti-Stokes frequency ω _CARS = ω _pump – ω _Stokes + ω _probe . The CARS signal ω _CARS is resonantly enhanced when the difference between the pump ω _pump and Stokes ω _Stokes frequencies matches a vibrational transition ω _vib of the molecule

Combining of coherent anti-Stokes Raman scattering (CARS) spectroscopy with the microscopy opens up unique method for chemical imaging. CARS microscopy permits vibrational imaging with high-sensitivity, high speed, and three-dimensional nearly diffraction limited spatial resolution.

Specifications

1) Measured 200 x 200 pixels.

2) Inquire for custom pulse repetition rates.

3) Measured at 800 nm.

4) Measured at 400 nm.

5) Pulse duration can vary depending on wavelength and pump energy.

6) Beam diameter at the FWHM level and can vary depending on the pump pulse energy.

7) Full angle measured at the FWHM level.

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